EC Broth with MUG is used to detect Escherichia coli in water, milk, and food. The presence of fluorescence using a long-wave UV light source confirms the presence of Escherichia coli and no further confirmation is required. MUG detects anaerogenic strains, which may not be detected in the conventional procedure. Lactose is a source of energy. Casein peptone provides additional nutrients. The mixture of bile salts is inhibiting for gram-positive bacteria, particularly bacilli and fecal streptococci. The substrate 4-methylumbelliferyl-b-Dglucuronide is hydrolyzed by an enzyme, b-glucuronidase, possessed by most Escherichia coli and a few strains of Salmonella, Shigella and Yersinia, to produce a fluorescent end product, 4methylumbelliferone.
Ready-to-use media considerably reduces the preparation time in quality control laboratories and also effectively reduces the risks of cross-contamination. GVS is cooperating closely with quality assurance managers in the industry in the development of its own media and test kits. This intensive product development has produced a range of products that is being used to monitor production plants and conduct microbiological checks on raw materials through to final product release in laboratories.
- Ready-to-use media reduces the preparation time in quality control laboratories
- Reduces the risks of cross-contamination
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